Dynamics of lactate dehydrogenase catalytic activity under thermomodification

  • Yu. V. Zimin Nizhny Novgorod Institute of Traumatology and Orthopedics, Russia
  • A. V. Bochkareva Nizhny Novgorod Institute of Traumatology and Orthopedics, Russia
  • A. G. Solovyeva Nizhny Novgorod Institute of Traumatology and Orthopedics, Russia
  • A. K. Martusevich Nizhny Novgorod Institute of Traumatology and Orthopedics, Russia
Keywords: Lactate dehydrogenase, thermodenaturation, spectroscopy

Abstract

Catalytic activity and kinetic characteristics of the rats (Wistar line) hepatic lactate dehydrogenase before and after thermodenaturation (15 minutes, 60°C) were studied. We found out that the enzyme thermodenaturation brings to activation of the direct and inhibition of the back lactate dehydrogenase reactions . This is confirmed by the enzyme activity rate and kinetic characteristics.

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Zimin Yu.V., Syatkin S.P., Berezov T.T. Metabolic adaptation molecular mechanisms of pathologically changed hepar under toxic hepatitis. Med. chem. quest. 2001; (3) 47: 346-352.

Dynamics of lactate dehydrogenase catalytic activity under thermomodification
Published
2008-05-05
How to Cite
Zimin, Y., Bochkareva, A., Solovyeva, A., & Martusevich, A. (2008). Dynamics of lactate dehydrogenase catalytic activity under thermomodification. Bulletin of the International Scientific Surgical Association, 3(1), 48-49. Retrieved from http://surgjournal.ru/index.php/BISSA/article/view/240
Section
Original Articles